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β-Tubulin (5G3) Mouse mAb (HRP)

-YM2208

2 5
主要信息
Target

Tubulin β

Host Species

Mouse

Reactivity

Human, Mouse, Rat, Mk, Dg, Ch, Hamster, Rabbit, sheep, Insect, Yeast

Applications

WB, IHC, IF

MW

55kD (Observed)

Conjugate/Modification

HRP

详细信息
推荐稀释比
WB 1:1000-1:3000; IHC 1:50-1:200; IF 1:200
Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
特异性
Theβ-tubulin antibody can detects endogenousβ-tubulin protein.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year (Do not lower than -25°C)
浓度
1 mg/ml
实测条带
55kD
偶联
HRP
克隆性
Monoclonal
克隆号
5G3
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抗原&靶点信息
免疫原:
Recombinant Protein of β-tubulin (HRP Conjugated)
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特异性:
Theβ-tubulin antibody can detects endogenousβ-tubulin protein.
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基因名称:
TUBB
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蛋白名称:
Tubulin beta chain (Tubulin beta-5 chain)
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别名:
Tubulin beta chain ;
Tubulin beta-5 chain ;
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数据库链接:
Organism 基因 ID SwissProt
Human 203068; P07437;
Mouse P99024;
Rat P69897;
背景:
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia , complex , with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1 , 6 , 7 , 8 , 9 , and 13. [provided by RefSeq , Jun 2014] ,
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功能:
Domain:The highly acidic C-terminal region may bind cations such as calcium. ,Function:Tubulin is the major constituent of microtubules. It binds two moles of GTP , one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. ,online information:Tubulin entry ,similarity:Belongs to the tubulin family. ,subunit:Dimer of alpha and beta chains. ,tissue specificity:Ubiquitously expressed with highest levels in spleen , thymus and immature brain. ,
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细胞定位:
Cytoplasm , cytoskeleton .
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研究领域:
>>Phagosome ;
>>Gap junction ;
>>Alzheimer disease ;
>>Parkinson disease ;
>>Amyotrophic lateral sclerosis ;
>>Huntington disease ;
>>Prion disease ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Pathogenic Escherichia coli infection ;
>>Salmonella infection
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