IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
特异性
Phospho-Syndecan-4 (S179) Polyclonal Antibody detects endogenous levels of Syndecan-4 protein only when phosphorylated at S179.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):EGsYD
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
理论分子量
22kD
修饰
Phospho
克隆性
Polyclonal
同种型
IgG
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抗原&靶点信息
免疫原:
The antiserum was produced against synthesized peptide derived from human Syndecan4 around the phosphorylation site of Ser179. AA range:145-194
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特异性:
Phospho-Syndecan-4 (S179) Polyclonal Antibody detects endogenous levels of Syndecan-4 protein only when phosphorylated at S179.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):EGsYD
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008],
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功能:
Function:Cell surface proteoglycan that bears heparan sulfate.,Function:Cell surface proteoglycan.,similarity:Belongs to the syndecan proteoglycan family.,subunit:Homodimer. Interacts with CDCP1, with GIPC PDZ domain and with NUDT16L1/SDOS (By similarity). Interacts with SDCBP.,tissue specificity:Expressed in epithelial and fibroblastic cells.,
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细胞定位:
[Isoform 1]: Membrane ; Single-pass type I membrane protein . Secreted . Shedding of the ectodomain produces a soluble form. .; [Isoform 2]: Secreted.
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组织表达:
Expressed in epithelial and fibroblastic cells.
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研究领域:
>>ECM-receptor interaction ;
>>Cell adhesion molecules ;
>>Proteoglycans in cancer ;
>>Fluid shear stress and atherosclerosis