The antiserum was produced against synthesized peptide derived from human PLD1 around the phosphorylation site of Ser561. AA range:527-576

Phospho-PC-PLD1 (S561) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at S561.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):KFsLY

PLD1

Phospholipase D1

PLD1 ;
Phospholipase D1 ;
PLD 1 ;
hPLD1 ;
Choline phosphatase 1 ;
Phosphatidylcholine-hydrolyzing phospholipase D1

This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011],

Catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,enzyme regulation:Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42. Inhibited by oleate.,Function:Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.,online information:Phospholipase D entry,similarity:Belongs to the phospholipase D family.,similarity:Contains 1 PH domain.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 PLD phosphodiesterase domains.,subunit:Interacts with PIP5K1A.,tissue specificity:Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.,

Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Late endosome membrane ; Lipid-anchor ; Cytoplasmic side .

Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.

>>Glycerophospholipid metabolism ;
>>Ether lipid metabolism ;
>>Metabolic pathways ;
>>Ras signaling pathway ;
>>cAMP signaling pathway ;
>>Sphingolipid signaling pathway ;
>>Phospholipase D signaling pathway ;
>>Endocytosis ;
>>Fc gamma R-mediated phagocytosis ;
>>Glutamatergic synapse ;
>>GnRH signaling pathway ;
>>Parathyroid hormone synthesis, secretion and action ;
>>Pathways in cancer ;
>>Chemical carcinogenesis - reactive oxygen species ;
>>Pancreatic cancer ;
>>Choline metabolism in cancer