Synthesized peptide derived from human NSUN2 AA range: 658-708

This antibody detects endogenous levels of NSUN2 at Human/Mouse

NSUN2 SAKI TRM4

NSUN2

tRNA ;
cytosine ;
34 ;
-C ;
5 ;
-methyltransferase ;
Myc-induced SUN domain-containing protein ;
Misu ;
NOL1/NOP2/Sun domain family member 2 ;
Substrate of AIM1/Aurora kinase B ;
tRNA ;
cytosine-5- ;
-methyltransferase ;
tRNA methyltransferase 4 homolog ;
hTrm4 ;

This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011],

Catalytic activity:S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.,Function:RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation.,PTM:Phosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.,similarity:Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.,subcellular location:Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis.,subunit:Interacts with NPM1 and NCL during interphase; interaction is disrupted following phosphorylation at Ser-139.,

Nucleus, nucleolus . Cytoplasm . Mitochondrion . Cytoplasm, cytoskeleton, spindle . Secreted, extracellular exosome . Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA (PubMed:19596847). In testis, localizes to the chromatoid body (By similarity). .

Expressed in adult and fetal brain and in lymphoblastoid cells.