The antiserum was produced against synthesized peptide derived from human p40 phox around the phosphorylation site of Thr154. AA range:120-169

Phospho-p40-phox (T154) Polyclonal Antibody detects endogenous levels of p40-phox protein only when phosphorylated at T154.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PRtRK

NCF4

Neutrophil cytosol factor 4

NCF4 ;
SH3PXD4 ;
Neutrophil cytosol factor 4 ;
NCF-4 ;
Neutrophil NADPH oxidase factor 4 ;
SH3 and PX domain-containing protein 4 ;
p40-phox ;
p40phox

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding d

Function:Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 1 SH3 domain.,subunit:p40-PHOX associates primarily with p67-PHOX to form a complex with p47-PHOX.,tissue specificity:Expression is restricted to hematopoietic cells.,

Cytoplasm, cytosol . Endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Membrane ; Peripheral membrane protein .

Expression is restricted to hematopoietic cells.

>>Phagosome ;
>>Osteoclast differentiation ;
>>Neutrophil extracellular trap formation ;
>>Leukocyte transendothelial migration ;
>>Prion disease ;
>>Leishmaniasis ;
>>Diabetic cardiomyopathy ;
>>Lipid and atherosclerosis