The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214

Cleaved-PARP-1 (D214) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to D214.

PARP1

Poly [ADP-ribose] polymerase 1

PARP1 ;
ADPRT ;
PPOL ;
Poly [ADP-ribose] polymerase 1 ;
PARP-1 ;
ADP-ribosyltransferase diphtheria toxin-like 1 ;
ARTD1 ;
NAD ;
+ ;
ADP-ribosyltransferase 1 ;
ADPRT 1 ;
Poly[ADP-ribose] synthase 1

This gene encodes a chromatin-associated enzyme , poly (ADP-ribosyl) transferase , which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation , proliferation , and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition , this enzyme may be the site of mutation in Fanconi anemia , and may participate in the pathophysiology of type I diabetes. [provided by RefSeq , Jul 2008] ,

Catalytic activity:NAD (+) + (ADP-D-ribosyl) (n) -acceptor = nicotinamide + (ADP-D-ribosyl) (n+1) -acceptor. ,Function:Involved in the base excision repair (BER) pathway , by catalyzing the poly (ADP-ribosyl) ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. ,miscellaneous:The ADP-D-ribosyl group of NAD (+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself , and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety , building up a polymer with an average chain length of 20-30 units. ,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage , probably by ATM or ATR. ,PTM:Poly-ADP-ribosylated by PARP2. ,similarity:Contains 1 BRCT domain. ,similarity:Contains 1 PARP alpha-helical domain. ,similarity:Contains 1 PARP catalytic domain. ,similarity:Contains 2 PARP-type zinc fingers. ,subunit:Component of a base excision repair (BER) complex , containing at least XRCC1 , PARP2 , POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3 , APTX and SRY. The SWAP complex consists of NPM1 , NCL , PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. ,

Nucleus . Nucleus , nucleolus . Chromosome . Localizes to sites of DNA damage. .

>>Base excision repair ;
>>NF-kappa B signaling pathway ;
>>Apoptosis ;
>>Necroptosis ;
>>Diabetic cardiomyopathy