Rad2/FEN1 (Phospho Ser187) Rabbit pAb
-YP1733
主要信息
Target
FEN-1 Phospho Ser187
Host Species
Rabbit
Reactivity
Human, Mouse, Rat
Applications
WB
MW
42kD (Observed)
Conjugate/Modification
Phospho
货号: YP1733
规格
价格
货期
数量
200μL
￥4,680.00
现货
0
100μL
￥2,800.00
现货
0
50μL
￥1,500.00
现货
0
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详细信息
推荐稀释比
WB 1:500-2000
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
特异性
This antibody detects endogenous levels of Rad2/FEN1 (Phospho-Ser187) at Human, Mouse,Rat.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):FGsPV
纯化工艺
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
储存
-15°C to -25°C/1 year (Do not lower than -25°C)
浓度
1 mg/ml
实测条带
42kD
修饰
Phospho
克隆性
Polyclonal
同种型
IgG
抗原&靶点信息
免疫原:
Synthesized peptide derived from human Rad2/FEN1 (Phospho-Ser187)
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特异性:
This antibody detects endogenous levels of Rad2/FEN1 (Phospho-Ser187) at Human, Mouse,Rat.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):FGsPV
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基因名称:
FEN1 RAD2
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蛋白名称:
Rad2/FEN1 (Phospho-Ser187)
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别名:
Flap endonuclease 1 ;
FEN-1 ;
DNase IV ;
Flap structure-specific endonuclease 1 ;
Maturation factor 1 ;
MF1 ;
hFEN-1 ;
FEN-1 ;
DNase IV ;
Flap structure-specific endonuclease 1 ;
Maturation factor 1 ;
MF1 ;
hFEN-1 ;
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背景:
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate , thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore , secondary structure can deter the protective function of this protein , leading to site-specific trinucleotide expansions
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功能:
cofactor:Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding. ,Function:Endonuclease that cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Also possesses 5' to 3' exonuclease activity on niked or gapped double-stranded DNA , and exhibits RNase H activity. ,PTM:Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300. ,similarity:Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily. ,subunit:Interacts with PCNA. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. ,
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细胞定位:
[Isoform 1]: Nucleus , nucleolus. Nucleus , nucleoplasm. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion .
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组织表达:
研究领域:
>>DNA replication ;
>>Base excision repair ;
>>Non-homologous end-joining
>>Base excision repair ;
>>Non-homologous end-joining
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文献引用（{{totalcount}}）
货号: YP1733
规格
价格
货期
数量
200μL
￥4,680.00
现货
0
100μL
￥2,800.00
现货
0
50μL
￥1,500.00
现货
0
加入购物车
已收藏
收藏
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