The antiserum was produced against synthesized peptide derived from human CAD around the phosphorylation site of Thr456. AA range:422-471

Phospho-CAD (T456) Polyclonal Antibody detects endogenous levels of CAD protein only when phosphorylated at T456.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PItPH

CAD

CAD protein

CAD ;
CAD protein

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II) , aspartate transcarbamoylase , and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade , which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq , Apr 2015] ,

Catalytic activity: (S) -dihydroorotate + H (2) O = N-carbamoyl-L-aspartate. ,Catalytic activity:2 ATP + L-glutamine + HCO (3) (-) + H (2) O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. ,Catalytic activity:Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. ,cofactor:Binds 1 zinc ion per subunit (for dihydroorotase activity) . ,enzyme regulation:Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction. ,Function:This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase , CPSase , ATCase and DHOase) . ,miscellaneous:GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5) . ,online information:Aspartate carbamoyltransferase entry ,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 1/6. ,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 2/6. ,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO (3) (-) : step 3/6. ,similarity:Belongs to the ATCase/OTCase family. ,similarity:Contains 1 glutamine amidotransferase type-1 domain. ,similarity:Contains 2 ATP-grasp domains. ,similarity:In the central section; belongs to the DHOase family. ,subunit:Homohexamer. ,

Cytoplasm . Nucleus . Cytosolic and unphosphorylated in resting cells , translocates to the nucleus in response to EGF stimulation , nuclear import promotes optimal cell growth.

>>Pyrimidine metabolism ;
>>Alanine , aspartate and glutamate metabolism ;
>>Metabolic pathways ;
>>Biosynthesis of cofactors