The antiserum was produced against synthesized peptide derived from human HP1 alpha around the phosphorylation site of Ser92. AA range:58-107

Phospho-HP1α (S92) Polyclonal Antibody detects endogenous levels of HP1α protein only when phosphorylated at S92.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RKsNF

CBX5 HP1A

Chromobox protein homolog 5 (Antigen p25) (Heterochromatin protein 1 homolog alpha) (HP1 alpha)

CBX5 ;
HP1A ;
Chromobox protein homolog 5 ;
Antigen p25 ;
Heterochromatin protein 1 homolog alpha ;
HP1 alpha

This gene encodes a highly conserved nonhistone protein , which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues , and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants , encoding the same protein , have been identified. [provided by RefSeq , Jul 2008] ,

Function:Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9' , leading to epigenetic repression. Can interact with lamin B receptor (LBR) . This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. ,PTM:Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity) . Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. ,similarity:Contains 2 chromo domains. ,subcellular location:Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. ,subunit:Interacts with SUV420H1 and SUV420H2 (By similarity) . Interacts directly with ATRX , CHAF1A , LBR , NIPBL , SP100 , STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127. Interacts with HP1BP3. ,

Nucleus . Chromosome . Chromosome , centromere . Colocalizes with HNRNPU in the nucleus (PubMed:19617346) . Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. .