The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr249. AA range:215-264

Phospho-p130 Cas (Y249) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y249.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):DIyDV

BCAR1

Breast cancer anti-estrogen resistance protein 1

BCAR1 ;
CAS ;
CASS1 ;
CRKAS ;
Breast cancer anti-estrogen resistance protein 1 ;
CRK-associated substrate ;
Cas scaffolding protein family member 1 ;
p130cas

BCAR1 , or CAS , is an Src (MIM 190090) family kinase substrate involved in various cellular events , including migration , survival , transformation , and invasion (Sawada et al. , 2006 [PubMed 17129785]) .[supplied by OMIM , May 2009] ,

Domain:A serine-rich region promotes activation of the serum response element (SRE) . ,Domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK , NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL. ,Domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1. ,Function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. ,PTM:Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix. ,similarity:Belongs to the CAS family. ,similarity:Contains 1 SH3 domain. ,subcellular location:Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation. ,subunit:Forms complexes in vivo with focal adhesion kinase 1 , adapter protein CRKL and LYN kinase. Can heterodimerize with CASL. Interacts with BCAR3 , NPHP1 , PTK2B and SH2D3C (By similarity) . Interacts with activated CSPG4. Interacts with INPPL1/SHIP2. ,tissue specificity:Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver , thymus , and peripheral blood leukocytes. The protein has been detected in a B-cell line. ,

Cell junction , focal adhesion . Cytoplasm . Cell projection , axon . Unphosphorylated form localizes in the cytoplasm (By similarity) . Localizes to focal adhesion sites following integrin engagement (By similarity) . .

>>Rap1 signaling pathway ;
>>Chemokine signaling pathway ;
>>Focal adhesion ;
>>Leukocyte transendothelial migration ;
>>Regulation of actin cytoskeleton ;
>>Growth hormone synthesis , secretion and action ;
>>Bacterial invasion of epithelial cells ;
>>Shigellosis ;
>>Yersinia infection ;
>>Human cytomegalovirus infection