The antiserum was produced against synthesized peptide derived from human PKCB around the phosphorylation site of Ser661. AA range:622-671

Phospho-PKC β (S661) Polyclonal Antibody detects endogenous levels of PKC β protein only when phosphorylated at S661.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):GFsYT

PRKCB

Protein kinase C beta type

PRKCB ;
PKCB ;
PRKCB1 ;
Protein kinase C beta type ;
PKC-B ;
PKC-beta

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters , a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions , such as B cell activation , apoptosis induction , endothelial cell proliferation , and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stres

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,cofactor:Binds 3 calcium ions per subunit. The ions are bound to the C2 domain. ,Function:This is a calcium-activated , phospholipid-dependent , serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters , a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking. ,PTM:Phosphorylation on Thr-500 of isoform beta-I , within the activation loop , renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence , and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly , isoform beta-II is autophosphorylated on 'Thr-640' and 'Ser-659' , subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on PKC activity. ,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. ,similarity:Contains 1 AGC-kinase C-terminal domain. ,similarity:Contains 1 C2 domain. ,similarity:Contains 1 protein kinase domain. ,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers. ,subunit:Interacts with PDK1 (By similarity) . Interacts in vitro with PRKCBP1. ,

Cytoplasm . Nucleus . Membrane ; Peripheral membrane protein .

>>EGFR tyrosine kinase inhibitor resistance ;
>>MAPK signaling pathway ;
>>ErbB signaling pathway ;
>>Ras signaling pathway ;
>>Rap1 signaling pathway ;
>>Calcium signaling pathway ;
>>Chemokine signaling pathway ;
>>NF-kappa B signaling pathway ;
>>HIF-1 signaling pathway ;
>>Phosphatidylinositol signaling system ;
>>Sphingolipid signaling pathway ;
>>mTOR signaling pathway ;
>>Vascular smooth muscle contraction ;
>>Wnt signaling pathway ;
>>VEGF signaling pathway ;
>>Focal adhesion ;
>>Gap junction ;
>>Neutrophil extracellular trap formation ;
>>Natural killer cell mediated cytotoxicity ;
>>B cell receptor signaling pathway ;
>>Fc gamma R-mediated phagocytosis ;
>>Leukocyte transendothelial migration ;
>>Circadian entrainment ;
>>Long-term potentiation ;
>>Retrograde endocannabinoid signaling ;
>>Glutamatergic synapse ;
>>Cholinergic synapse ;
>>Serotonergic synapse ;
>>GABAergic synapse ;
>>Dopaminergic synapse ;
>>Long-term depression ;
>>Inflammatory mediator regulation of TRP channels ;
>>Insulin secretion ;
>>GnRH signaling pathway ;
>>Melanogenesis ;
>>Thyroid hormone synthesis ;
>>Thyroid hormone signaling pathway ;
>>Oxytocin signaling pathway ;
>>Aldosterone synthesis and secretion ;
>>Parathyroid hormone synthesis , secretion and action ;
>>GnRH secretion ;
>>Insulin resistance ;
>>AGE-RAGE signaling pathway in diabetic complications ;
>>Growth hormone synthesis , secretion and action ;
>>Aldosterone-regulated sodium reabsorption ;
>>Endocrine and other factor-regulated calcium reabsorption ;
>>Salivary secretion ;
>>Gastric acid secretion ;
>>Pancreatic secretion ;
>>Carbohydrate digestion and absorption ;
>>Spinocerebellar ataxia ;
>>Pathways of neurodegeneration - multiple diseases ;
>>Amphetamine addiction ;
>>Morphine addiction ;
>>Leishmaniasis ;
>>African trypanosomiasis ;
>>Amoebiasis ;
>>Hepatitis B ;
>>Human cytomegalovirus infection ;
>>Influenza A ;
>>Human immunodeficiency virus 1 infection ;
>>Coronavirus disease - COVID-19 ;
>>Pathways in cancer ;
>>Proteoglycans in cancer ;
>>MicroRNAs in cancer ;
>>Chemical carcinogenesis - receptor activation ;
>>Glioma ;
>>Non-small cell lung cancer ;
>>Hepatocellular carcinoma ;
>>Choline metabolism in cancer ;
>>Diabetic cardiomyopathy