The antiserum was produced against synthesized peptide derived from human Werner Syndrome Helicase around the phosphorylation site of Ser1141. AA range:1107-1156

Phospho-WRN (S1141) Polyclonal Antibody detects endogenous levels of WRN protein only when phosphorylated at S1141.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):SSsQP

WRN RECQ3 RECQL2

Werner syndrome ATP-dependent helicase

WRN ;
RECQ3 ;
RECQL2 ;
Werner syndrome ATP-dependent helicase ;
DNA helicase ;
RecQ-like type 3 ;
RecQ3 ;
Exonuclease WRN ;
RecQ protein-like 2

Werner syndrome RecQ like helicase (WRN) Homo sapiens This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism , including transcription , replication , recombination , and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity , and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing , this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome , an autosomal recessive disorder characterized by premature aging. [provided by RefSeq , Jul 2008] ,

Disease:Defects in WRN are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is a rare autosomal recessive progeroid syndrome characterized by the premature onset of multiple age-related disorders , including atherosclerosis , cancer , non-insulin-dependent diabetes mellitus , ocular cataracts and osteoporosis. The major cause of death , at a median age of 47 , is myocardial infarction. Currently all known WS mutations produces prematurely terminated proteins. ,Disease:Defects in WRN may be a cause of colorectal cancer (CRC) [MIM:114500]. ,Function:Essential for the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity. May be involved in the control of genomic stability. ,online information:WRN mutation db (Warner disease) ,PTM:Phosphorylated by PRKDC. Phosphorylated upon DNA damage , probably by ATM or ATR. ,similarity:Belongs to the helicase family. RecQ subfamily. ,similarity:Contains 1 3'-5' exonuclease domain. ,similarity:Contains 1 helicase ATP-binding domain. ,similarity:Contains 1 helicase C-terminal domain. ,similarity:Contains 1 HRDC domain. ,subunit:Interacts via its N-terminal domain with WRNIP1 (By similarity) . Interacts with EXO1. ,

Nucleus , nucleolus . Nucleus . Nucleus , nucleoplasm . Chromosome . Gamma-irradiation leads to its translocation from nucleoli to nucleoplasm and PML regulates the irradiation-induced WRN relocation (PubMed:21639834) . Localizes to DNA damage sites (PubMed:27063109) . .