Synthesized peptide derived from human ZAK (Phospho-Ser165)

This antibody detects endogenous levels of ZAK (Phospho-Ser165) at Human, Mouse,Rat.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):HMsLV

MLTK ZAK HCCS4

ZAK (Phospho-Ser165)

Mitogen-activated protein kinase kinase kinase MLT ;
Human cervical cancer suppressor gene 4 protein ;
HCCS-4 ;
Leucine zipper- and sterile alpha motif-containing kinase ;
MLK-like mitogen-activated protein triple kinase ;
Mixed lineage kinase-related kinase ;
MLK-related kinase ;
MRK ;
Sterile alpha motif- and leucine zipper-containing kinase AZK ;

This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation by PKN1 and autophosphorylation on Thr-161 and Ser-165.,Function:Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,subcellular location:Translocates to the nucleus upon ultraviolet B irradiation.,subunit:Homodimer. Interacts with PKN1 and ZNF33A.,tissue specificity:Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.,

Cytoplasm . Nucleus . Translocates to the nucleus upon ultraviolet B irradiation. .

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