GAPDH (2B8) Mouse mAb (HRP)
-YM2054
主要信息
Target
GAPDH
Host Species
Mouse
Reactivity
Human, Mouse, Rat, Mk, Dg, Ch, Hamster, Pig, sheep, Insect, Yeast
Applications
WB, IF, IHC
MW
36kD (Calculated)
Conjugate/Modification
HRP
货号: YM2054
规格
价格
货期
数量
500μL
￥4,200.00
两周
0
100μL
￥960.00
两周
0
50μL
￥560.00
两周
0
加入购物车
已收藏
收藏
偶联抗体
GAPDH (2B8) Mouse mAb (AbFluor 350)
GAPDH (2B8) Mouse mAb (AbFluor 405)
GAPDH (2B8) Mouse mAb (AbFluor 488)
GAPDH (2B8) Mouse mAb (AbFluor 555)
GAPDH (2B8) Mouse mAb (AbFluor 594)
GAPDH (2B8) Mouse mAb (AbFluor 647)
GAPDH (2B8) Mouse mAb (AbFluor 680)
GAPDH (2B8) Mouse mAb (Biotin)
GAPDH (2B8) Mouse mAb (Cy3)
GAPDH (2B8) Mouse mAb (Cy5)
GAPDH (2B8) Mouse mAb (FITC)
详细信息
推荐稀释比
Optimal working dilutions should be determined experimentally by the investigator; Suggested starting dilutions are as follows:WB 1:5000; IHC 1:200.
Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
组成
Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.
特异性
GAPDH Monoclonal Antibody(2B8) specially designed for your immunoassay as internal control.
纯化工艺
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
储存
Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
浓度
1mg/ml
理论分子量
36kD
偶联
HRP
克隆性
Monoclonal
克隆号
2B8
同种型
IgG1
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抗原&靶点信息
免疫原:
The specific immunogen used to produce this antibody is proprietary information.
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特异性:
GAPDH Monoclonal Antibody(2B8) specially designed for your immunoassay as internal control.
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基因名称:
GAPDH
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蛋白名称:
Glyceraldehyde-3-phosphate dehydrogenase
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别名:
GAPDH
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背景:
glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism , the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also , this protein contains a peptide that has antimicrobial activity against E. coli , P. aeruginosa , and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins , the regulation of mRNA stability , and acting as a transferri
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功能:
Catalytic activity:D-glyceraldehyde 3-phosphate + phosphate + NAD (+) = 3-phospho-D-glyceroyl phosphate + NADH. ,Function:Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretory pathway. ,online information:Glyceraldehyde 3-phosphate dehydrogenase entry ,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1. ,pathway:Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. ,PTM:Reversible S-nitrosylation of Cys-152 inhibits enzymatic activity and increases endogenous ADP-ribosylation , which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis. ,sequence Caution:Differs quite extensively. ,similarity:Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. ,subcellular location:Postnuclear and Perinuclear regions. ,subunit:Homotetramer. ,subunit:Homotetramer. Binds PRKCI. ,
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细胞定位:
Cytoplasm , cytosol . Nucleus . Cytoplasm , perinuclear region . Membrane . Cytoplasm , cytoskeleton . Translocates to the nucleus following S-nitrosylation and interaction with SIAH1 , which contains a nuclear localization signal (By similarity) . Postnuclear and Perinuclear regions (PubMed:12829261) . .
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研究领域:
>>Glycolysis / Gluconeogenesis ;
>>Metabolic pathways ;
>>Carbon metabolism ;
>>Biosynthesis of amino acids ;
>>HIF-1 signaling pathway ;
>>Alzheimer disease ;
>>Pathogenic Escherichia coli infection ;
>>Salmonella infection ;
>>Diabetic cardiomyopathy
>>Metabolic pathways ;
>>Carbon metabolism ;
>>Biosynthesis of amino acids ;
>>HIF-1 signaling pathway ;
>>Alzheimer disease ;
>>Pathogenic Escherichia coli infection ;
>>Salmonella infection ;
>>Diabetic cardiomyopathy
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信号通路
文献引用（{{totalcount}}）
货号: YM2054
规格
价格
货期
数量
500μL
￥4,200.00
两周
0
100μL
￥960.00
两周
0
50μL
￥560.00
两周
0
加入购物车
已收藏
收藏
偶联抗体
GAPDH (2B8) Mouse mAb (AbFluor 350)
GAPDH (2B8) Mouse mAb (AbFluor 405)
GAPDH (2B8) Mouse mAb (AbFluor 488)
GAPDH (2B8) Mouse mAb (AbFluor 555)
GAPDH (2B8) Mouse mAb (AbFluor 594)
GAPDH (2B8) Mouse mAb (AbFluor 647)
GAPDH (2B8) Mouse mAb (AbFluor 680)
GAPDH (2B8) Mouse mAb (Biotin)
GAPDH (2B8) Mouse mAb (Cy3)
GAPDH (2B8) Mouse mAb (Cy5)
GAPDH (2B8) Mouse mAb (FITC)
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