The antiserum was produced against synthesized peptide derived from human PKMYT1. AA range:49-98

Myt 1 Polyclonal Antibody detects endogenous levels of Myt 1 protein.

PKMYT1

Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase

PKMYT1 ;
MYT1 ;
Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase ;
Myt1 kinase

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.,enzyme regulation:Negatively regulated by hyperphosphorylation during mitosis. The hyperphosphorylated form does not associate with CCNB1-CDC2 complexes. The PLK1 protein kinase may be required for mitotic phosphorylation.,Function:Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the cdc2 kinase specifically when cdc2 is complexed to cyclins. Mediates phosphorylation of cdc2 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.,PTM:Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.,sequence Caution:Chimeric cDNA.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.,

Endoplasmic reticulum membrane ; Peripheral membrane protein . Golgi apparatus membrane ; Peripheral membrane protein .

Brain,Epithelium,PCR rescued clones,

>>Cell cycle ;
>>Oocyte meiosis ;
>>Progesterone-mediated oocyte maturation