The antiserum was produced against synthesized peptide derived from human Cytochrome P450 3A4. AA range:91-140

CYP3A4 Polyclonal Antibody detects endogenous levels of CYP3A4 protein.

CYP3A4

Cytochrome P450 3A4

CYP3A4 ;
CYP3A3 ;
Cytochrome P450 3A4 ;
1 ;
8-cineole 2-exo-monooxygenase ;
Albendazole monooxygenase ;
Albendazole sulfoxidase ;
CYPIIIA3 ;
CYPIIIA4 ;
Cytochrome P450 3A3 ;
Cytochrome P450 HLp ;
Cytochrome P450 NF-25 ;
Cytochrome P450-PCN1 ;
Nifedipine

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isofor

Catalytic activity:Albendazole + NADPH + O(2) = albendazole S-oxide + NADP(+) + H(2)O.,Catalytic activity:Lithocholate + NADPH + O(2) = hyodeoxycholate + NADP(+) + H(2)O.,Catalytic activity:Quinine + NADPH + O(2) = 3-hydroxyquinine + NADP(+) + H(2)O.,Catalytic activity:Taurochenodeoxycholate + NADPH + O(2) = taurohyocholate + NADP(+) + H(2)O.,cofactor:Heme group.,Function:Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.,induction:By glucocorticoids. Also induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.,online information:CYP3A4 alleles,online information:CYP3A4 entry,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Expressed in prostate and liver.,

Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane ; Single-pass membrane protein.

Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).

>>Steroid hormone biosynthesis ;
>>Linoleic acid metabolism ;
>>Retinol metabolism ;
>>Metabolism of xenobiotics by cytochrome P450 ;
>>Drug metabolism - cytochrome P450 ;
>>Drug metabolism - other enzymes ;
>>Metabolic pathways ;
>>Bile secretion ;
>>Chemical carcinogenesis - DNA adducts ;
>>Chemical carcinogenesis - receptor activation