Synthesized peptide derived from human OGT (phospho Thr454)

This antibody detects endogenous levels of Human,Mouse,Rat OGT (phospho Thr454).The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):YRtAL

OGT

OGT (phospho Thr454)

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit ;
O-GlcNAc transferase subunit p110 ;
O-linked N-acetylglucosamine transferase 110 kDa subunit ;
OGT ;

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2009],

Catalytic activity:UDP-N-acetyl-D-glucosamine + peptide = UDP + N-acetyl-beta-D-glucosaminyl-peptide.,Function:Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine.,online information:UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110kDa subunit,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the O-GlcNAc transferase family.,similarity:Contains 13 TPR repeats.,subunit:Heterotrimer of two 110 kDa and one 70 kDa subunits. It is not known if the 70 kDa subunit is encoded by a separate gene or is the product of either of a proteolytic degradation or an alternative initiation of the 110 kDa subunit (By similarity). Interacts with HCFC1.,tissue specificity:Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.,

Nucleus . Cytoplasm . Predominantly localizes to the nucleus. .; [Isoform 2]: Mitochondrion . Membrane . Associates with the mitochondrial inner membrane. .; [Isoform 3]: Cytoplasm . Nucleus . Cell membrane . Mitochondrion membrane . Cell projection . Mostly in the nucleus. Retained in the nucleus via interaction with HCFC1 (PubMed:21285374). After insulin induction, translocated from the nucleus to the cell membrane via phosphatidylinositide binding. Colocalizes with AKT1 at the plasma membrane. TRAK1 recruits this protein to mitochondria. In the absence of TRAK1, localizes in cytosol and nucleus (By similarity). .; [Isoform 4]: Cytoplasm. Nucleus.

Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

>>Other types of O-glycan biosynthesis ;
>>Insulin resistance