The antiserum was produced against synthesized peptide derived from human MYPT1 around the phosphorylation site of Thr696. AA range:661-710

Phospho-MYPT1 (T696) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T696.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RStQG

PPP1R12A

Protein phosphatase 1 regulatory subunit 12A

PPP1R12A ;
MBS ;
MYPT1 ;
Protein phosphatase 1 regulatory subunit 12A ;
Myosin phosphatase-targeting subunit 1 ;
Myosin phosphatase target subunit 1 ;
Protein phosphatase myosin-binding subunit

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosph

Function:Regulates myosin phosphatase activity.,PTM:Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 6 ANK repeats.,subcellular location:Along actomyosin filaments and stress fibers.,subunit:PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.,

Cytoplasm . Cytoplasm, cytoskeleton, stress fiber . Also along actomyosin filaments. .

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

>>cGMP-PKG signaling pathway ;
>>cAMP signaling pathway ;
>>Vascular smooth muscle contraction ;
>>Focal adhesion ;
>>Platelet activation ;
>>Regulation of actin cytoskeleton ;
>>Oxytocin signaling pathway ;
>>Proteoglycans in cancer