The antiserum was produced against synthesized peptide derived from human MLC-2 around the phosphorylation site of Thr17 and Ser18. AA range:5-54

This antibody detects endogenous levels of MLC-2 only when phosphorylated at Thr17 or Ser18,and dually phosphorylated at two sites.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):AtsNV

MYL12A

Myosin regulatory light chain 12A

MYL12A ;
MLCB ;
MRLC3 ;
RLC ;
Myosin regulatory light chain 12A ;
MLC-2B ;
Myosin RLC ;
Myosin regulatory light chain 2 ;
nonsarcomeric ;
Myosin regulatory light chain MRLC3 ;
MYL12B ;
MRLC2 ;
MYLC2B ;
Myosin regulatory light chain 12B ;
MLC-2A ;
MLC-2 ;
M

This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.[provided by RefSeq, Dec 2014],

Function:Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.,miscellaneous:This chain binds calcium.,PTM:Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains.,

stress fiber,cytosol,brush border,myosin complex,myosin II complex,Z disc,apical part of cell,extracellular exosome,

Coronary artery,Epithelium,Placenta,Skeletal muscle,Skin,Testis,

>>Axon guidance ;
>>Focal adhesion ;
>>Tight junction ;
>>Platelet activation ;
>>Leukocyte transendothelial migration ;
>>Regulation of actin cytoskeleton ;
>>Shigellosis ;
>>Salmonella infection