Purified recombinant human AIF protein fragments expressed in E.coli.

This antibody detects endogenous levels of AIF and does not cross-react with related proteins.

AIFM1 ALF PDCD8

Apoptosis-inducing factor 1 mitochondrial

AIFM1 ;
AIF ;
PDCD8 ;
Apoptosis-inducing factor 1 ;
mitochondrial ;
Programmed cell death protein 8

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome

Catalytic activity:2 glutathione + protein-disulfide = glutathione disulfide + protein-dithiol.,cofactor:FAD.,Function:Possesses significant protein thiol-disulfide oxidase activity.,Function:Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro). Binds to DNA in a sequence-independent manner.,similarity:Belongs to the FAD-dependent oxidoreductase family.,similarity:Contains 1 thioredoxin domain.,subcellular location:Translocated to the nucleus upon induction of apoptosis.,subunit:Interacts with XIAP.,tissue specificity:Widely expressed.,

Mitochondrion intermembrane space . Mitochondrion inner membrane. Cytoplasm . Nucleus . Cytoplasm, perinuclear region . Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis (PubMed:15775970). Colocalizes with EIF3G in the nucleus and perinuclear region (PubMed:17094969). .; [Isoform 3]: Mitochondrion intermembrane space . Mitochondrion inner membrane . Has a stronger membrane anchorage than isoform 1. .; [Isoform 4]: Mitochondrion . Cytoplasm, cytosol . In pro-apoptotic conditions, is released from mitochondria to cytosol in a calpain/cathepsin-dependent manner. .; [Isoform 5]: Cytoplasm .

Expressed in all tested tissues (PubMed:16644725). Detected in muscle and skin fibroblasts (at protein level) (PubMed:23217327). Expressed in osteoblasts (at protein level) (PubMed:28842795). ; [Isoform 3]: Brain specific. ; [Isoform 4]: Expressed in all tested tissues except brain. ; [Isoform 5]: Isoform 5 is frequently down-regulated in human cancers.

>>Apoptosis ;
>>Necroptosis