WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications. Note:For IHC,wesuggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
组成
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
特异性
Nap1 Polyclonal Antibody detects endogenous levels of Nap1 protein.
纯化工艺
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
浓度
1 mg/ml
实测条带
48kD
修饰
Unmodified
克隆性
Polyclonal
同种型
IgG
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抗原&靶点信息
免疫原:
The antiserum was produced against synthesized peptide derived from human AZI2. AA range:10-59
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特异性:
Nap1 Polyclonal Antibody detects endogenous levels of Nap1 protein.
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基因名称:
AZI2
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蛋白名称:
5-azacytidine-induced protein 2
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别名:
AZI2 ;
NAP1 ;
5-azacytidine-induced protein 2 ;
NF-kappa-B-activating kinase-associated protein 1 ;
Nak-associated protein 1 ;
TILP
AZI2, or NAP1, contributes to the activation of NFKB (see MIM 164011)-dependent gene expression by activating IKK-related kinases, such as NAK (TBK1; MIM 604834) (Fujita et al., 2003 [PubMed 14560022]).[supplied by OMIM, Mar 2008],
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功能:
Function:Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFN-beta promoter activation via TICAM1.,subunit:Interacts with IKBKE, TBK1 and TICAM1.,tissue specificity:Widely expressed. Abundant expression seen in the pancreas and testis.,
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细胞定位:
Cytoplasm .
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组织表达:
Widely expressed (PubMed:14560022). Abundant expression seen in the pancreas and testis (PubMed:14560022).