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DDDDK-Tag(binds to flag sequnence) (12M11) Mouse mAb

-YM3808

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主要信息
Target

Flag-tag

Host Species

Mouse

Reactivity

Speciesindependent

Applications

WB, ELISA, IP, IF, CoIP

Conjugate/Modification

Unmodified

详细信息
推荐稀释比
WB 1:10000-50000; ELISA 1:20000-50000; IP 1:50-1:200; IF 1:1000 -3000
组成
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
来源
Mouse VH IgG1 and VL Kappa was expressed from 293F cells
特异性
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
纯化工艺
Protein A purified
纯度
>95% SDS-PAGE
储存
-15°C to -25°C/1 year(Do not lower than -25°C)
修饰
Unmodified
克隆性
Monoclonal
克隆号
12M11
同种型
VH IgG1 and VL Kappa
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抗原&靶点信息
免疫原:
Synthetic Peptide of Flag-Tag
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特异性:
This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa.
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基因名称:
Flag tag; Flag-tag,DDDDK TAG, DDDDK-TAG, DYKDDDDK tag,DYKDDDDK-tag
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背景:
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
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