CASP3

Caspase-3 ;
CASP-3 ;
Apopain ;
Cysteine protease CPP32 ;
CPP-32 ;
Protein Yama ;
SREBP cleavage activity 1 ;
SCA-1 ;
[Cleaved into: Caspase-3 subunit p17 ;
Caspase-3 subunit p12]

catalytic activity:Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3 , although Val or Ala are also accepted at this position. ,enzyme regulation:Inhibited by isatin sulfonamides. ,function:Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6 , -7 and -9. Involved in the cleavage of huntingtin. ,PTM:Cleavage by granzyme B , caspase-6 , caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. ,PTM:S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway , associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits , but also by stimulating the denitrosylation of its active site thiol. ,similarity:Belongs to the peptidase C14A family. ,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers , each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. ,tissue specificity:Highly expressed in lung , spleen , heart , liver and kidney. Moderate levels in brain and skeletal muscle , and low in testis. Also found in many cell lines , highest expression in cells of the immune system. ,

regulation of cyclin-dependent protein kinase activity , DNA catabolic process , endonucleolytic , leukocyte homeostasis ,B cell homeostasis , release of cytochrome c from mitochondria , lymphocyte homeostasis , negative regulation of immune system process , regulation of leukocyte activation , negative regulation of leukocyte activation , DNA metabolic process , DNA catabolic process , DNA fragmentation involved in apoptosis , negative regulation of protein kinase activity , proteolysis , apoptosis , induction of apoptosis , cell structure disassembly during apoptosis , response to DNA damage stimulus , nucleus organization , mitochondrion organization , ectoderm development , heart development ,sensory perception , sensory perception of sound , cell death , negative regulation of cell proliferation , epidermis development , induction of apoptosis by extracellular signals , induction of apoptosis by intracellular signals , induction of apoptosis by oxidative stress , apoptotic mitochondrial changes , macromolecule catabolic process , response to radiation , response to UV , response to light stimulus , response to wounding , response to abiotic stimulus , epidermal cell differentiation , response to organic substance , regulation of cell death , positive regulation of cell death ,programmed cell death , induction of programmed cell death , death , regulation of phosphate metabolic process ,cellular component disassembly , keratinocyte differentiation , apoptotic nuclear changes , nuclear fragmentation during apoptosis , epithelial cell differentiation , regulation of B cell proliferation , negative regulation of B cell proliferation ,regulation of mononuclear cell proliferation , negative regulation of mononuclear cell proliferation , cellular response to stress , negative regulation of kinase activity , response to cytokine stimulus , response to tumor necrosis factor ,regulation of cell proliferation , regulation of T cell proliferation , negative regulation of T cell proliferation , regulation of phosphorylation , homeostatic process , regulation of apoptosis , T cell homeostasis , positive regulation of apoptosis ,negative regulation of apoptosis , regulation of programmed cell death , positive regulation of programmed cell death ,negative regulation of programmed cell death , negative regulation of catalytic activity , regulation of kinase activity ,negative regulation of molecular function , cellular macromolecule catabolic process , cell fate commitment , negative regulation of cyclin-dependent protein kinase activity , negative regulation of cell cycle , regulation of protein kinase activity , regulation of activated T cell proliferation , negative regulation of activated T cell proliferation , homeostasis of number of cells , regulation of lymphocyte proliferation , negative regulation of lymphocyte proliferation , regulation of T cell activation , regulation of B cell activation , regulation of cell activation , negative regulation of cell activation ,negative regulation of T cell activation , negative regulation of B cell activation , neurological system process , cognition ,sensory perception of mechanical stimulus , regulation of phosphorus metabolic process , regulation of lymphocyte activation , negative regulation of lymphocyte activation , regulation of transferase activity , negative regulation of transferase activity , neuron apoptosis , regulation of cell cycle , epithelium development , negative regulation of cell death , regulation of leukocyte proliferation , negative regulation of leukocyte proliferation ,

Cytoplasm.