ARHGEF2

Rho guanine nucleotide exchange factor 2 ;
Guanine nucleotide exchange factor H1 ;
GEF-H1 ;
Microtubule-regulated Rho-GEF ;
Proliferating cell nucleolar antigen p40 ;

domain:The DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA. ,domain:The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions. ,function:Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability , cell motility and polarization , dendritic spine morphology , antigen presentation , leukemic cell differentiation , cell cycle regulation , and cancer. Binds Rac-GTPases , but does not seem to promote nucleotide exchange activity toward Rac-GTPases , which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42 , TC10 , or Ras-GTPases. ,online information:ARHGEF2 entry ,PTM:Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta , promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis , which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules. ,sequence caution:Sequence differs at a large extent from the sequence shown in the paper. ,similarity:Contains 1 DH (DBL-homology) domain. ,similarity:Contains 1 PH domain. ,similarity:Contains 1 phorbol-ester/DAG-type zinc finger. ,subcellular location:Localizes to the tips of cortical microtubules of the mitotic spindle during cell division , and is further released upon microtubule depolymerization. ,subunit:Interacts with 14-3-3 zeta; when phosphorylated at Ser-886. Interacts with the kinases PAK4 , AURKA/STK6 and MAPK1. Interacts with RHOA and RAC1. ,

M phase of mitotic cell cycle , mitotic cell cycle , M phase , nuclear division , cell morphogenesis , intracellular protein transport , apoptosis , induction of apoptosis , cytoskeleton organization , actin filament organization , negative regulation of microtubule depolymerization , cell cycle , mitosis , intracellular signaling cascade , protein localization , cell death , induction of apoptosis by extracellular signals , negative regulation of organelle organization , regulation of cell death , positive regulation of cell death , programmed cell death , induction of programmed cell death , protein transport ,death , cell cycle process , cell cycle phase , actin filament-based process , actin cytoskeleton organization , regulation of microtubule polymerization or depolymerization , negative regulation of microtubule polymerization or depolymerization , regulation of microtubule depolymerization , regulation of microtubule-based process , cellular component morphogenesis , regulation of organelle organization , cellular protein localization , regulation of Rho protein signal transduction , regulation of cell proliferation , regulation of apoptosis , positive regulation of apoptosis , regulation of programmed cell death , positive regulation of programmed cell death , negative regulation of protein complex disassembly , regulation of protein complex disassembly , establishment of protein localization , regulation of Ras protein signal transduction , intracellular transport , organelle fission , regulation of small GTPase mediated signal transduction , negative regulation of cellular component organization , cell division , regulation of cytoskeleton organization , negative regulation of cytoskeleton organization , regulation of microtubule cytoskeleton organization ,cellular macromolecule localization ,

Cytoplasm , cytoskeleton . Cytoplasm . Cell junction , tight junction . Golgi apparatus . Cytoplasm , cytoskeleton , spindle . Cell projection , ruffle membrane . Cytoplasmic vesicle . Localizes to the tips of cortical microtubules of the mitotic spindle during cell division , and is further released upon microtubule depolymerization (PubMed:15827085) . Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells (PubMed:19043560) . Colocalized with NOD2 and RIPK2 in vesicles and with the cytoskeleton (PubMed:21887730) . .