Detects endogenous levels of Erk8 when phosphorylated at Thr175 or Tyr177, and dually phosphorylated at two sites

MAPK15

Mitogen-activated protein kinase 15 ;
MAP kinase 15 ;
MAPK 15 ;
Extracellular signal-regulated kinase 7 ;
ERK-7 ;
Extracellular signal-regulated kinase 8 ;
ERK-8 ;

catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation. ,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. ,enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases , such as DUSP1. ,function:In vitro , phosphorylates MBP. ,PTM:Dually phosphorylated on Thr-175 and Tyr-177 , which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. ,PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. ,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. ,similarity:Contains 1 protein kinase domain. ,subunit:Interacts with CSK/c-Src , ABL1 , RET and TGFB1I1. ,tissue specificity:Widely expressed with a maximal expression in lung and kidney. ,

regulation of protein amino acid phosphorylation , positive regulation of protein amino acid phosphorylation , regulation of DNA replication , protein amino acid phosphorylation , phosphorus metabolic process , phosphate metabolic process ,negative regulation of DNA replication , response to endogenous stimulus , response to hormone stimulus , negative regulation of biosynthetic process , regulation of catabolic process , positive regulation of catabolic process , response to organic substance , negative regulation of macromolecule biosynthetic process , positive regulation of phosphorus metabolic process , positive regulation of macromolecule metabolic process , negative regulation of macromolecule metabolic process , phosphorylation , regulation of phosphate metabolic process , negative regulation of cellular biosynthetic process , regulation of protein ubiquitination , positive regulation of protein ubiquitination , regulation of protein modification process , positive regulation of protein modification process , regulation of cellular protein metabolic process , positive regulation of cellular protein metabolic process , response to estradiol stimulus , regulation of protein catabolic process , regulation of phosphorylation , positive regulation of phosphorylation , response to estrogen stimulus , positive regulation of protein catabolic process , negative regulation of nucleobase , nucleoside , nucleotide and nucleic acid metabolic process , positive regulation of phosphate metabolic process , protein amino acid autophosphorylation , response to steroid hormone stimulus , regulation of DNA metabolic process , negative regulation of DNA metabolic process , negative regulation of nitrogen compound metabolic process , regulation of phosphorus metabolic process , positive regulation of protein metabolic process ,

Cytoplasm , cytoskeleton , cilium basal body . Cell junction , tight junction . Cytoplasm , cytoskeleton , microtubule organizing center , centrosome , centriole . Cytoplasmic vesicle , autophagosome . Golgi apparatus . Nucleus . Cytoplasm . Cytoplasm , cytoskeleton , spindle . Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936) . Translocates to the nucleus upon activation (PubMed:20638370) . At prometaphase I , metaphase I (MI) , anaphase I , telophase I , and metaphase II (MII) stages , is stably detected at the spindle (By similarity) . .