Amino acid: 521-627, with his-MBP tag.

ACC1 α protein

catalytic activity:ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA.,catalytic activity:ATP + biotin-carboxyl-carrier protein + CO(2) = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.,cofactor:Binds 2 manganese ions per subunit.,cofactor:Biotin.,enzyme regulation:Activated by citrate. Inhibited by malonyl-CoA.,function:ACC-beta may be involved in the provision of malonyl-CoA or in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.,pathway:Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.,similarity:Contains 1 ATP-grasp domain.,similarity:Contains 1 biotin carboxylation domain.,similarity:Contains 1 biotinyl-binding domain.,similarity:Contains 1 carboxyltransferase domain.,subcellular location:May associate with membranes.,tissue specificity:Predominantly expressed in the heart, skeletal muscles and liver.,

fatty acid metabolic process, fatty acid biosynthetic process, lipid biosynthetic process, regulation of cellular ketone metabolic process, organic acid biosynthetic process, regulation of lipid metabolic process, regulation of fatty acid metabolic process, regulation of fatty acid oxidation, carboxylic acid biosynthetic process,

Mitochondrion .

Widely expressed with highest levels in heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland and colon (PubMed:9099716). Isoform 3 is expressed in skeletal muscle, adipose tissue and liver (at protein level) (PubMed:19190759). Isoform 3 is detected at high levels in adipose tissue with lower levels in heart, liver, skeletal muscle and testis (PubMed:19190759).