PPP2CA

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform ;
PP2A-alpha ;
Replication protein C ;
RP-C ;

catalytic activity:A phosphoprotein + H (2) O = a protein + phosphate. ,cofactor:Binds 1 iron ion per subunit. ,cofactor:Binds 1 manganese ion per subunit. ,function:PP2A can modulate the activity of phosphorylase B kinase casein kinase 2 , mitogen-stimulated S6 kinase , and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen , preferentially on serine residues 120 , 123 , 677 , and perhaps 679. The C subunit was most active , followed by the AC form , which was more active than the ABC form , and activity of all three forms was strongly stimulated by manganese , and to a lesser extent by magnesium. Dephosphorylation by the AC form , but not C or ABC form is inhibited by small T antigen. ,PTM:Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. ,PTM:Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. ,similarity:Belongs to the PPP phosphatase family. ,similarity:Belongs to the PPP phosphatase family. PP-1 subfamily. ,subcellular location:In prometaphase cells , but not in anaphase cells , localizes at centromeres. During mitosis , also found at spindle poles. ,subunit:PP2A consists of a common heterodimeric core enzyme , composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A) , that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55 , R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) , the 48 kDa variable regulatory subunit , viral proteins , and cell signaling molecules. Interacts with NXN; the interaction is direct (By similarity) . May indirectly interact with SGOL1 , most probably through regulatory B56 subunits. ,

MAPKKK cascade , inactivation of MAPK activity , regulation of cell growth , regulation of protein amino acid phosphorylation , negative regulation of protein amino acid phosphorylation , regulation of DNA replication , RNA processing , negative regulation of protein kinase activity , protein amino acid dephosphorylation , membrane lipid metabolic process , sphingolipid metabolic process , ceramide metabolic process , phosphorus metabolic process ,phosphate metabolic process , induction of apoptosis , intracellular signaling cascade , protein kinase cascade ,mesoderm development , regulation of cell size , RNA splicing , negative regulation of signal transduction , response to organic substance , negative regulation of phosphorus metabolic process , negative regulation of macromolecule metabolic process , regulation of protein kinase cascade , negative regulation of cell communication , negative regulation of protein kinase cascade , regulation of cell death , positive regulation of cell death , induction of programmed cell death , dephosphorylation , regulation of phosphate metabolic process , second-messenger-mediated signaling , regulation of Wnt receptor signaling pathway , regulation of cell adhesion , negative regulation of cell growth ,regulation of protein modification process , negative regulation of protein modification process , regulation of cellular protein metabolic process , negative regulation of cellular protein metabolic process , regulation of cellular component size , negative regulation of kinase activity , regulation of growth , regulation of phosphorylation , negative regulation of phosphorylation , regulation of tyrosine phosphorylation of STAT protein , regulation of tyrosine phosphorylation of Stat3 protein , negative regulation of tyrosine phosphorylation of Stat3 protein , negative regulation of tyrosine phosphorylation of STAT protein , regulation of apoptosis , positive regulation of apoptosis , regulation of programmed cell death , positive regulation of programmed cell death , negative regulation of catalytic activity , regulation of MAP kinase activity , negative regulation of MAP kinase activity , regulation of kinase activity , negative regulation of molecular function , regulation of transcription , negative regulation of cell size , regulation of protein kinase activity ,negative regulation of growth , negative regulation of phosphate metabolic process , regulation of JAK-STAT cascade ,negative regulation of JAK-STAT cascade , sphingoid metabolic process , regulation of peptidyl-tyrosine phosphorylation ,negative regulation of peptidyl-tyrosine phosphorylation , regulation of DNA metabolic process , regulation of phosphorus metabolic process , negative regulation of protein metabolic process , regulation of transferase activity ,negative regulation of transferase activity ,

Cytoplasm . Nucleus . Chromosome , centromere . Cytoplasm , cytoskeleton , spindle pole . In prometaphase cells , but not in anaphase cells , localizes at centromeres (PubMed:16541025) . During mitosis , also found at spindle poles (PubMed:16541025) . Centromeric localization requires the presence of SGO2 (By similarity) . .