catalytic activity:Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7 , this enzyme cleaves type III collagen more slowly than type I. ,cofactor:Binds 2 zinc ions per subunit. ,cofactor:Binds 3 calcium ions per subunit. ,domain:The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion , thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. ,enzyme regulation:Cannot be activated without removal of the activation peptide. ,function:Can degrade fibrillar type I , II , and III collagens. ,similarity:Belongs to the peptidase M10A family. ,similarity:Contains 4 hemopexin-like domains. ,subcellular location:Stored in intracellular granules. ,tissue specificity:Neutrophils. ,
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