Detects endogenous levels of Erk8 when phosphorylated at Thr175 or Tyr177, and dually phosphorylated at two sites

MAPK15

Mitogen-activated protein kinase 15 ;
MAP kinase 15 ;
MAPK 15 ;
Extracellular signal-regulated kinase 7 ;
ERK-7 ;
Extracellular signal-regulated kinase 8 ;
ERK-8 ;

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1.,function:In vitro, phosphorylates MBP.,PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro.,PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1.,tissue specificity:Widely expressed with a maximal expression in lung and kidney.,

regulation of protein amino acid phosphorylation, positive regulation of protein amino acid phosphorylation, regulation of DNA replication, protein amino acid phosphorylation, phosphorus metabolic process, phosphate metabolic process,negative regulation of DNA replication, response to endogenous stimulus, response to hormone stimulus, negative regulation of biosynthetic process, regulation of catabolic process, positive regulation of catabolic process, response to organic substance, negative regulation of macromolecule biosynthetic process, positive regulation of phosphorus metabolic process, positive regulation of macromolecule metabolic process, negative regulation of macromolecule metabolic process, phosphorylation, regulation of phosphate metabolic process, negative regulation of cellular biosynthetic process, regulation of protein ubiquitination, positive regulation of protein ubiquitination, regulation of protein modification process, positive regulation of protein modification process, regulation of cellular protein metabolic process, positive regulation of cellular protein metabolic process, response to estradiol stimulus, regulation of protein catabolic process, regulation of phosphorylation, positive regulation of phosphorylation, response to estrogen stimulus, positive regulation of protein catabolic process, negative regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process, positive regulation of phosphate metabolic process, protein amino acid autophosphorylation, response to steroid hormone stimulus, regulation of DNA metabolic process, negative regulation of DNA metabolic process, negative regulation of nitrogen compound metabolic process, regulation of phosphorus metabolic process, positive regulation of protein metabolic process,

Cytoplasm, cytoskeleton, cilium basal body . Cell junction, tight junction . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasmic vesicle, autophagosome . Golgi apparatus . Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936). Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity). .

Widely expressed with a maximal expression in lung and kidney.