Synthesized peptide derived from human EKLF (Acetyl Lys274)

This antibody detects endogenous levels of Human EKLF (Acetyl Lys274).The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):ARkRQ

KLF1 EKLF

EKLF (Acetyl Lys274)

Krueppel-like factor 1 ;
Erythroid krueppel-like transcription factor ;
EKLF ;

This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In (Lu) blood phenotype. [provided by RefSeq , Oct 2009] ,

Function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation , acts as a Probably serves as a general switch factor for erythroid development. When sumoylated , acts as a transcriptional repressor , by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation. ,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity. ,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity. ,PTM:Sumoylated; sumoylation , promoted by PIAS1 , leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex. ,similarity:Belongs to the krueppel C2H2-type zinc-finger protein family. ,similarity:Contains 3 C2H2-type zinc fingers. ,subcellular location:Colocalizes with SUMO1 in nuclear speckles. ,subunit:Interacts with CBP and EP300; the interactions enhance the transactivation activity. Interacts with PCAF; the interaction does not acetylate EKLF and inhibits its transactivation activity. ,tissue specificity:Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines. ,

Nucleus . Colocalizes with SUMO1 in nuclear speckles. .