Synthesized acetyl-peptide derived from the human NF-E4 around the acetylation site of K43.

Acetyl-NF-E4 (K43) Polyclonal AntibodySynthesized peptide derived from the human NF-E4 around the acetylation site of K43.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PRkQA

NFE4

Transcription factor NF-E4

NFE4 ;
Transcription factor NF-E4

The erythroid-specific protein encoded by this gene , and the ubiquitous transcription factor CP2 , form the stage selector protein (SSP) complex , which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon , and a downstream AUG codon , results in two isoforms. While the long isoform (22 kDa) acts as an activator , the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq , Jul 2008] ,

Function:Functions as part of the SSP (stage selector protein) complex , a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR) . The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast , isoform 2 acts as a repressor of gamma-globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter. ,PTM:Acetylation at Lys-43 prolongs the protein half-life by preventing ubiquitin-mediated degradation and reduces the interaction between NF-E4 and HDAC1 , potentially maximizing the activating ability of the factor at the gamma-promoter. ,PTM:Ubiquitinated; leading to its degradation by the proteasome. Acetylation at Lys-43 prevents ubiquitination. ,sequence Caution:Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon. ,subunit:Component of the SSP (stage selector protein) complex , which appears to be a heteromer of TFCP2 and 2 copies of NFE4. Interacts with HDAC1 and PCAF. Isoform 2 interacts with TFCP2. ,tissue specificity:Specifically expressed in fetal liver , cord blood and bone marrow. Also expressed in the K562 and HEL cell lines , which constitutively express the fetal globin genes. ,

Nucleus .