Synthesized peptide derived from human PDE4D (Phospho-Ser578)

This antibody detects endogenous levels of PDE4D (Phospho-Ser578) at Human, Mouse,Rat.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PQsPS

PDE4D DPDE3

PDE4D (Phospho-Ser578)

cAMP-specific 3',5'-cyclic phosphodiesterase 4D ;
DPDE3 ;
PDE43 ;

This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3' ,5'-cyclic-AMP phosphodiesterase activity and degrades cAMP , which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq , Sep 2009] ,

Catalytic activity:Adenosine 3' ,5'-cyclic phosphate + H (2) O = adenosine 5'-phosphate. ,cofactor:Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions , while site 2 has a preference for magnesium and/or manganese ions. ,Disease:Genetic variations in PDE4D might be associated with susceptibility to stroke type 1 (STRK1) [MIM:606799]. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor , sensory and/or cognitive function. PubMed:17006457 states that association with stroke has to be considered with caution. ,enzyme regulation:Inhibited by rolipram. Activated by phosphatidic acid. ,Function:Regulates the levels of cAMP in the cell. ,pathway:Purine metabolism; cAMP degradation; AMP from cAMP: step 1/1. ,PTM:Isoform 2 and isoform 11 are activated by phosphorylation (in vitro) , but not isoform 8. Isoform 7 and isoform 12 are phosphorylated on Ser-49 , Ser-51 , Ser-55 and Ser-59. ,similarity:Belongs to the cyclic nucleotide phosphodiesterase family. ,subcellular location:Found in the soluble fraction , associated with membranes , and associated with the cytoskeleton and the centrosome. ,subunit:Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 2 is part of a ternary complex containing PRKAR2A , PRKAR2B and AKAP9. Interacts with PDE4DIP (By similarity) . Isoform 5 binds GNB2L1 via its unique N-terminus. Binds ARRB2. ,tissue specificity:Widespread; most abundant in skeletal muscle. Isoform 8 is detected in brain. Isoform 9 is detected in brain , placenta , lung and kidney. Isoform 11 is detected in heart and skeletal muscle. ,

Apical cell membrane . Cytoplasm . Membrane . Cytoplasm , cytoskeleton . Cytoplasm , cytoskeleton , microtubule organizing center , centrosome . Found in the soluble fraction , associated with membranes , and associated with the cytoskeleton and the centrosome (By similarity) . Colocalized with SHANK2 to the apical membrane of colonic crypt cells. .

>>Purine metabolism ;
>>Metabolic pathways ;
>>cAMP signaling pathway ;
>>Parathyroid hormone synthesis , secretion and action ;
>>Morphine addiction