Synthesized peptide derived from human INCENP (Phospho-Thr59)

This antibody detects endogenous levels of INCENP (Phospho-Thr59) at Human, Mouse,Rat.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PKtPS

INCENP

Inner centromere protein

Inner centromere protein

In mammalian cells , 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger ,' or transiently interacting , proteins (reviewed by Choo , 1997) . The constitutive proteins include CENPA (centromere protein A; MIM 117139) , CENPB (MIM 117140) , CENPC1 (MIM 117141) , and CENPD (MIM 117142) . The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay , 1994 [PubMed 8088460]) . These include CENPE (MIM 117143) ; MCAK (MIM 604538) ; KID (MIM 603213) ; cytoplasmic dynein (e.g. , MIM 600112) ; CliPs (e.g. , MIM 179838) ; and CENPF/mitosin (MIM 600236) . The inner centromere proteins (INCENPs) (Earnshaw and Cooke , 1991 [PubMed 1860899]) , the initial members of the passenger protein group , display a broad localization alo

Caution:PubMed:11139336 experiments have been carried out partly in chicken and partly in human. ,Function:Component of the chromosomal passenger complex (CPC) , a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. ,similarity:Belongs to the INCENP family. ,subcellular location:Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. ,subunit:Homodimer or heterodimer. Interacts with H2AFZ (By similarity) . Interacts with CBX3. Interacts with tubulin beta chain. Interacts with AURKB and AURKC. Component of the CPC at least composed of BIRC5/survivin , CDCA8/borealin , INCENP and AURKB/Aurora-B. Interacts with EVI5. ,

Nucleus . Chromosome , centromere . Cytoplasm , cytoskeleton , spindle . Midbody . Chromosome , centromere , kinetochore . Colocalized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters. Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores , in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone (By similarity) . Colocalizes with AURKB at mitotic chromosomes (PubMed:11453556) . Localizes to inner kinetochore (PubMed:16760428) . Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis (PubMed:15316025) . Cocalizes to the equatorial cell cortex at anaphase (PubMed:11453556) . .