Synthesized peptide derived from human XRCC4 (Phospho-Ser260)

This antibody detects endogenous levels of XRCC4 (Phospho-Ser260) at Human, Mouse,Rat.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):ISsLD

XRCC4

XRCC4 (Phospho-Ser260)

DNA repair protein XRCC4 ;
X-ray repair cross-complementing protein 4 ;

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V (D) J recombination. Mutations in this gene can cause short stature , microcephaly , and endocrine dysfunction (SSMED) . Alternative splicing generates several transcript variants. [provided by RefSeq , Dec 2015] ,

Function:Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D) J recombination. Binds to DNA and to DNA ligase IV (LIG4) . The LIG4-XRCC4 complex is responsible for the NHEJ ligation step , and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. ,PTM:Monoubiquitinated. ,PTM:Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX. ,PTM:Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination. ,similarity:Belongs to the XRCC4 family. ,subunit:Homodimer and homotetramer in solution. The homodimer associates with LIG4 , and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF. ,tissue specificity:Widely expressed. ,

Nucleus . Chromosome . Localizes to site of double-strand breaks. .; [Protein XRCC4 , C-terminus]: Cytoplasm . Translocates from the nucleus to the cytoplasm following cleavage by caspase-3 (CASP3) . .

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