Synthesized phosho peptide around human SIRT1 (Ser27)

This antibody detects endogenous levels of Human SIRT1 (phospho-Ser27)

SIRT1 SIR2L1

SirT1 (Ser27)

NAD-dependent protein deacetylase sirtuin-1 ;
hSIRT1 ;
Regulatory protein SIR2 homolog 1 ;
SIR2-like protein 1 ;
hSIR2 ;
[Cleaved into: SirtT1 75 kDa fragment ;
75SirT1 ;
]

This gene encodes a member of the sirtuin family of proteins , homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however , yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq , Dec 2008] ,

Catalytic activity:NAD (+) + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. ,cofactor:Binds 1 zinc ion per subunit. ,enzyme regulation:Inhibited by nicotinamide. Activated by resveratrol (3 ,5 ,4'-trihydroxy-trans-stilbene) , butein (3 ,4 ,2' ,4'-tetrahydroxychalcone) , piceatannol (3 ,5 ,3' ,4'-tetrahydroxy-trans-stilbene) , Isoliquiritigenin (4 ,2' ,4'-trihydroxychalcone) , fisetin (3 ,7 ,3' ,4'-tetrahydroxyflavone) and quercetin (3 ,5 ,7 ,3' ,4'-pentahydroxyflavone) . RPS19BP1/AROS acts as a positive regulator of deacetylation activity. ,Function:NAD-dependent deacetylase , which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD (+) /NADH , which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro , such activity is either weak or inexistent in vivo. In case of HIV-1 infection , interacts with and deacetylates the viral Tat protein. ,miscellaneous:Red wine , which contains resveratrol , may participate in activation of sirtuin proteins , and may therefore participate in an extended lifespan as it has been observed in yeast. ,similarity:Belongs to the sirtuin family. ,similarity:Contains 1 deacetylase sirtuin-type domain. ,subcellular location:Recruited to the nuclear bodies via its interaction with PML. ,subunit:Interacts with TAF1B. Found in a complex with PCAF and MYOD1 (By similarity) . Interacts with MLLT7/FOXO4 , HES1 , HEY2 , p53/TP53 and PML. Interacts with RPS19BP1/AROS. ,tissue specificity:Widely expressed. ,

Nucleus , PML body . Cytoplasm . Nucleus . Recruited to the nuclear bodies via its interaction with PML (PubMed:12006491) . Colocalized with APEX1 in the nucleus (PubMed:19934257) . May be found in nucleolus , nuclear euchromatin , heterochromatin and inner membrane (PubMed:15469825) . Shuttles between nucleus and cytoplasm (By similarity) . Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178) . .; [SirtT1 75 kDa fragment]: Cytoplasm . Mitochondrion .

>>Nicotinate and nicotinamide metabolism ;
>>Metabolic pathways ;
>>FoxO signaling pathway ;
>>AMPK signaling pathway ;
>>Longevity regulating pathway ;
>>Longevity regulating pathway - multiple species ;
>>Cellular senescence ;
>>Glucagon signaling pathway ;
>>Alcoholic liver disease ;
>>Amphetamine addiction ;
>>MicroRNAs in cancer