Synthesized phosho peptide around human PRK1 (Thr774) and PRK2 (Thr816)

This antibody detects endogenous levels of Human Mouse Rat PRK1 (phospho-Thr774) or PRK2 (phospho-Thr816).The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RTStF

PRK1 (Thr774) /PRK2 (Thr816)

Serine/threonine-protein kinase N1 ;
Protease-activated kinase 1 ;
PAK-1 ;
Protein kinase C-like 1 ;
Protein kinase C-like PKN ;
Protein kinase PKN-alpha ;
Protein-kinase C-related kinase 1 ;
Serine-threonine protein kinase N ;

protein kinase N1 (PKN1) Homo sapiens The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase , which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq , Jul 2008] ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,Domain:The C1 domain does not bind the diacylglycerol (DAG) . ,enzyme regulation:Activated by lipids , particularly cardiolipin and to a lesser extent by other acidic phospholipids. Two specific sites , Thr-774 (activation loop of the kinase domain) and Ser-916 (turn motif) , need to be phosphorylated for its full activation. ,Function:Can phosphorylate ribosomal protein S6. Mediates GTPase Rho dependent intracellular signaling. ,PTM:Activated by limited proteolysis with trypsin. ,PTM:Autophosphorylated; preferably on serine. ,similarity:Belongs to the protein kinase superfamily. ,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. ,similarity:Contains 1 AGC-kinase C-terminal domain. ,similarity:Contains 1 C2 domain. ,similarity:Contains 1 protein kinase domain. ,similarity:Contains 3 REM (Hr1) repeats. ,subunit:Interacts with ZA20D3 (By similarity) . Interacts with RhoA and Rac1. ,tissue specificity:Found ubiquitously. Expressed in heart , brain , placenta , lung , skeletal muscle , kidney and pancreas. ,

Cytoplasm . Nucleus . Endosome . Cell membrane ; Peripheral membrane protein . Cleavage furrow . Midbody . Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. .

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