Synthesized phosho peptide around human NEDD4L (Ser448)

This antibody detects endogenous levels of Human NEDD4L (phospho-Ser448)

NEDD4L KIAA0439 NEDL3

NEDD4L (Ser448)

E3 ubiquitin-protein ligase NEDD4-like ;
NEDD4.2 ;
Nedd4-2 ;

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates , thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel , ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq , Mar 2012] ,

Function:E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC , Nav1.2 , Nav1.3 , Nav1.5 , Nav1.7 , Nav1.8 , Kv1.3 , EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK. ,induction:By androgens in prostate , and by albumin in kidney. ,pathway:Protein modification; protein ubiquitination. ,PTM:Phosphorylated by SGK or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation. ,similarity:Contains 1 C2 domain. ,similarity:Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. ,similarity:Contains 4 WW domains. ,subunit:Interacts with NDFIP2 and UBE2E3 (By similarity) . Interacts via its WW domains with SCNN1A , SCNN1B , SCNN1G , SCN1A , SCN2A , SCN3A , SCN5A , SCN8A , SCN9A , SCN10A and CLCN5. Interacts with SMAD2 , SMAD3 , SMAD6 and SMAD7. The phosphorylated form interacts with 14-3-3 proteins. Interacts with Epstein-Barr virus LMP2A. Interacts with NDFIP1 in vitro. ,tissue specificity:Ubiquitously expressed , with highest levels in prostate , pancreas and kidney. ,

Cytoplasm . Golgi apparatus . Endosome , multivesicular body . May be recruited to exosomes by NDFIP1.

>>Ubiquitin mediated proteolysis ;
>>Endocytosis ;
>>Tight junction ;
>>Aldosterone-regulated sodium reabsorption