The antiserum was produced against synthesized peptide derived from human MARK2 around the phosphorylation site of Thr596. AA range:562-611

Phospho-MARK2 (T596) Polyclonal Antibody detects endogenous levels of MARK2 protein only when phosphorylated at T596.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RStFH

MARK2

Serine/threonine-protein kinase MARK2

MARK2 ;
EMK1 ;
Serine/threonine-protein kinase MARK2 ;
ELKL motif kinase 1 ;
EMK-1 ;
MAP/microtubule affinity-regulating kinase 2 ;
PAR1 homolog ;
PAR1 homolog b ;
Par-1b ;
Par1b

microtubule affinity regulating kinase 2 (MARK2) Homo sapiens This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells , and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Jul 2009] ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,cofactor:Magnesium. ,enzyme regulation:Activated by phosphorylation on Thr-208 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. ,Function:Role in epithelial morphogenesis. Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells. One or more isoforms may play a role in graft rejection. ,similarity:Belongs to the protein kinase superfamily. ,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. ,similarity:Contains 1 KA1 (kinase-associated) domain. ,similarity:Contains 1 protein kinase domain. ,similarity:Contains 1 UBA domain. ,subcellular location:Phosphorylated by PRKCZ in polarized epithelial cells , resulting in an interaction with YWHAZ which promotes relocation from the lateral to the apical membrane. ,tissue specificity:High levels of expression in heart , brain , skeletal muscle and pancreas , lower levels observed in lung , liver and kidney. ,

Cell membrane; Peripheral membrane protein. Cytoplasm. Lateral cell membrane. Cytoplasm , cytoskeleton. Cell projection , dendrite . Cytoplasm . Phosphorylation at Thr-596 by PRKCZ/aPKC and subsequent interaction with 14-3-3 protein YWHAZ promotes relocation from the cell membrane to the cytoplasm.