Synthesized phosho peptide around human CAD (Ser1859)

This antibody detects endogenous levels of Human Mouse Rat CAD (phospho-Ser1859)

CAD

CAD (Ser1859)

CAD protein [Includes: Glutamine-dependent carbamoyl-phosphate synthase ;
Aspartate carbamoyltransferase ;
Dihydroorotase ;
]

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015],

Catalytic activity:(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.,Catalytic activity:2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.,Catalytic activity:Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.,cofactor:Binds 1 zinc ion per subunit (for dihydroorotase activity) .,enzyme regulation:Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction.,Function:This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).,miscellaneous:GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5).,online information:Aspartate carbamoyltransferase entry,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6.,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 2/6.,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.,similarity:Belongs to the ATCase/OTCase family.,similarity:Contains 1 glutamine amidotransferase type-1 domain.,similarity:Contains 2 ATP-grasp domains.,similarity:In the central section; belongs to the DHOase family.,subunit:Homohexamer.,

Cytoplasm . Nucleus . Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.

>>Pyrimidine metabolism ;
>>Alanine, aspartate and glutamate metabolism ;
>>Metabolic pathways ;
>>Biosynthesis of cofactors