The antiserum was produced against synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266. AA range:281-330

This antibody detects endogenous levels of MLK1/2 only when phosphorylated at Human:T312/266, Mouse:T305/266..The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):AGtYA

MAP3K9/MAP3K10

Mitogen-activated protein kinase kinase kinase 9/10

MAP3K9 ;
MLK1 ;
PRKE1 ;
Mitogen-activated protein kinase kinase kinase 9 ;
Mixed lineage kinase 1 ;
MAP3K10 ;
MLK2 ;
MST ;
Mitogen-activated protein kinase kinase kinase 10 ;
Mixed lineage kinase 2 ;
Protein kinase MST

catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,cofactor:Magnesium. ,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation. ,function:Activates the JUN N-terminal pathway. ,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. ,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. ,similarity:Contains 1 protein kinase domain. ,similarity:Contains 1 SH3 domain. ,subunit:Homodimer. ,tissue specificity:Expressed in epithelial tumor cell lines of colonic , breast and esophageal origin. ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,cofactor:Magnesium. ,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation. ,Function:Activates the JUN N-terminal pathway. ,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. ,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. ,similarity:Contains 1 protein kinase domain. ,similarity:Contains 1 SH3 domain. ,subunit:Homodimer. ,tissue specificity:Expressed in epithelial tumor cell lines of colonic , breast and esophageal origin. ,

intracellular ,integral component of membrane ,