The antiserum was produced against synthesized peptide derived from human CXCR4 around the phosphorylation site of Ser339. AA range:303-352

Phospho-Fusin (S339) Polyclonal Antibody detects endogenous levels of Fusin protein only when phosphorylated at S339.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):HSsVS

CXCR4

C-X-C chemokine receptor type 4

CXCR4 ;
C-X-C chemokine receptor type 4 ;
CXC-R4 ;
CXCR-4 ;
FB22 ;
Fusin ;
HM89 ;
LCR1 ;
Leukocyte-derived seven transmembrane domain receptor ;
LESTR ;
NPYRL ;
Stromal cell-derived factor 1 receptor ;
SDF-1 receptor ;
CD antigen CD184

C-X-C motif chemokine receptor 4 (CXCR4) Homo sapiens This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts , hypogammaglobulinemia , infections , and myelokathexis) syndrome. Alternate transcriptional splice variants , encoding different isoforms , have been characterized. [provided by RefSeq , Jul 2008] ,

Alternative products:Additional isoforms seem to exist ,Caution:Was originally (PubMed:8329116 and PubMed:8234909) thought to be a receptor for neuropeptide Y type 3 (NPY3R) (NPY3-R) . ,Disease:Defects in CXCR4 are a cause of WHIM syndrome [MIM:193670]; also called warts , hypogammaglobulinemia , infections , and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia , hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia , bone marrow aspirates from affected individuals contain abundant mature myeloid cells , a condition termed myelokathexis. ,Domain:The amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity. ,Function:Receptor for the C-X-C chemokine CXCL12/SDF-1. Transduces a signal by increasing the intracellular calcium ions level. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract , probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS , could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus. ,online information:CXC chemokine receptors entry ,online information:CXCR4 entry ,online information:CXCR4 mutation db ,PTM:O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity. ,PTM:Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization. ,similarity:Belongs to the G-protein coupled receptor 1 family. ,subunit:Monomer. Can form dimers. Interacts with HIV-1 surface protein gp120 and Tat. ,tissue specificity:Expressed in numerous tissues , such as peripheral blood leukocytes , spleen , thymus , spinal cord , heart , placenta , lung , liver , skeletal muscle , kidney , pancreas , cerebellum , cerebral cortex and medulla (in microglia as well as in astrocytes) , brain microvascular , coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested. ,

Cell membrane ; Multi-pass membrane protein . Cell junction. Early endosome. Late endosome. Lysosome. In unstimulated cells , diffuse pattern on plasma membrane. On agonist stimulation , colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen , distributes to the immunological synapse forming at the T-cell-APC contact area , where it localizes at the peripheral and distal supramolecular activation cluster (SMAC) .

>>Viral life cycle - HIV-1 ;
>>Calcium signaling pathway ;
>>Cytokine-cytokine receptor interaction ;
>>Viral protein interaction with cytokine and cytokine receptor ;
>>Chemokine signaling pathway ;
>>Endocytosis ;
>>Axon guidance ;
>>Leukocyte transendothelial migration ;
>>Intestinal immune network for IgA production ;
>>Regulation of actin cytoskeleton ;
>>Human cytomegalovirus infection ;
>>Human immunodeficiency virus 1 infection ;
>>Pathways in cancer