The antiserum was produced against synthesized peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99. AA range:65-114

Phospho-BLM (T99) Polyclonal Antibody detects endogenous levels of BLM protein only when phosphorylated at T99.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):QEtQR

BLM

Bloom syndrome protein

BLM ;
RECQ2 ;
RECQL3 ;
Bloom syndrome protein ;
DNA helicase ;
RecQ-like type 2 ;
RecQ2 ;
RecQ protein-like 3

The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq , Jul 2008] ,

Disease:Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency , sun-sensitive telangiectatic hypo- and hyperpigmented skin , predisposition to malignancy , and chromosomal instability. ,Function:Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. ,online information:BLM mutation db ,PTM:Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex , as well as the presence of RMI1. ,similarity:Belongs to the helicase family. RecQ subfamily. ,similarity:Contains 1 helicase ATP-binding domain. ,similarity:Contains 1 helicase C-terminal domain. ,similarity:Contains 1 HRDC domain. ,subunit:Part of the BRCA1-associated genome surveillance complex (BASC) , which contains BRCA1 , MSH2 , MSH6 , MLH1 , ATM , BLM , PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2. Interacts with RMI complex. Interacts directly with RMI1 component of RMI complex. ,

Nucleus . Together with SPIDR , is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner.

>>Homologous recombination ;
>>Fanconi anemia pathway