The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser142. AA range:111-160

Phospho-CREB-1 (S142) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S142.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):DLsSD

CREB1

Cyclic AMP-responsive element-binding protein 1

CREB1 ;
Cyclic AMP-responsive element-binding protein 1 ;
CREB-1 ;
cAMP-responsive element-binding protein 1

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element , an octameric palindrome. The protein is phosphorylated by several protein kinases , and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq , Mar 2016] ,

Disease:A chromosomal aberration involving CREB1 is associated with angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. Translocation t (2;22) (q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. ,Function:This protein binds the cAMP response element (CRE) , a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transciption activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity. ,PTM:Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity) . Phosphorylated upon DNA damage , probably by ATM or ATR. ,similarity:Belongs to the bZIP family. ,similarity:Contains 1 bZIP domain. ,similarity:Contains 1 KID (kinase-inducible) domain. ,subunit:Interacts with PPRC1. Binds DNA as a dimer. This dimer is stabilized by magnesium ions. Interacts , through the bZIP domain , with the coactivators TORC1/CRTC1 , TORC2/CRTC2 and TORC3/CRTC3. When phosphorylated on Ser-133 , binds CREBBP. ,

Nucleus .

>>cGMP-PKG signaling pathway ;
>>cAMP signaling pathway ;
>>PI3K-Akt signaling pathway ;
>>AMPK signaling pathway ;
>>Longevity regulating pathway ;
>>Adrenergic signaling in cardiomyocytes ;
>>Osteoclast differentiation ;
>>Antigen processing and presentation ;
>>TNF signaling pathway ;
>>Circadian rhythm ;
>>Circadian entrainment ;
>>Thermogenesis ;
>>Cholinergic synapse ;
>>Dopaminergic synapse ;
>>Insulin secretion ;
>>Estrogen signaling pathway ;
>>Melanogenesis ;
>>Thyroid hormone synthesis ;
>>Glucagon signaling pathway ;
>>Renin secretion ;
>>Aldosterone synthesis and secretion ;
>>Relaxin signaling pathway ;
>>Cortisol synthesis and secretion ;
>>Parathyroid hormone synthesis , secretion and action ;
>>Insulin resistance ;
>>Cushing syndrome ;
>>Growth hormone synthesis , secretion and action ;
>>Vasopressin-regulated water reabsorption ;
>>Huntington disease ;
>>Prion disease ;
>>Cocaine addiction ;
>>Amphetamine addiction ;
>>Alcoholism ;
>>Tuberculosis ;
>>Hepatitis B ;
>>Human cytomegalovirus infection ;
>>Human papillomavirus infection ;
>>Human T-cell leukemia virus 1 infection ;
>>Kaposi sarcoma-associated herpesvirus infection ;
>>Viral carcinogenesis ;
>>Chemical carcinogenesis - receptor activation ;
>>Prostate cancer