The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. AA range:24-73

Phospho-14-3-3 ζ (S58) Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein only when phosphorylated at S58.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RSsWR

YWHAZ

14-3-3 protein zeta/delta

YWHAZ ;
14-3-3 protein zeta/delta ;
Protein kinase C inhibitor protein 1 ;
KCIP-1

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals , and this protein is 99% identical to the mouse , rat and sheep orthologs. The encoded protein interacts with IRS1 protein , suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq , Oct 2008] ,

Caution:Was originally (PubMed:1577711) thought to have phospholipase A2 activity. ,Function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners , usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. ,PTM:The delta , brain-specific form differs from the zeta form in being phosphorylated (By similarity) . Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. ,similarity:Belongs to the 14-3-3 family. ,subcellular location:Located to stage I to stage IV melanosomes. ,subunit:Homodimer. Heterodimerizes with YWHAE. Homo- and hetero-dimerization is inhibited by phosphorylation on Ser-58. Interacts with FOXO4 , NOXA1 , SSH1 and ARHGEF2. Interacts with PCTK1 and BSPRY (By similarity) . Interacts with WEE1 (C-terminal) (By similarity) . Interacts with MLF1 (phosphorylated form) ; the interaction retains it in the cytoplasm (By similarity) . Interacts with Thr-phosphorylated ITGB2 (By similarity) . Interacts with Pseudomonas aeruginosa exoS (unphosphorylated form) . Interacts with BAX; the interaction occurs in the cytoplasm. Under stress conditions , MAPK8-mediated phosphorylation releases BAX to mitochondria. Interacts with phosphorylated RAF1; the interaction is inhibited when YWHAZ is phosphorylated on Thr-232. Interacts with TP53; the interaction enhances p53 transcriptional acivity. The Ser-58 phosphorylated form inhibits this interaction and p53 transcriptional activity. Interacts with ABL1 (phosphorylated form) ; the interaction retains ABL1 in the cytoplasm. Interacts with AANAT ('Thr-31' phosphorylated form) ; the interaction modulates AANAT enzymatic activity through preventing dephosphorylation and/or proteolysis and stabilizing substrate binding. Subsequently , a second molecule of AANAT ('Ser-205' phosphorylated form) , can bind the other YWHAZ monomer with similar effect. Interacts with AKT1; the interaction phosphorylates YWHAZ and modulates dimerization. ,

Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.

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