The antiserum was produced against synthesized peptide derived from human MKP-1/2 around the phosphorylation site of Ser296/318. AA range:261-310

This antibody detects endogenous levels of MKP-1/2 only when phosphorylated at Human:S296/318, Mouse:S296/322, Rat:S296/319..The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):IIsPN

DUSP1/4

Dual specificity protein phosphatase 1/4

DUSP1 ;
CL100 ;
MKP1 ;
PTPN10 ;
VH1 ;
Dual specificity protein phosphatase 1 ;
Dual specificity protein phosphatase hVH1 ;
Mitogen-activated protein kinase phosphatase 1 ;
MAP kinase phosphatase 1 ;
MKP-1 ;
Protein-tyrosine phosphatase CL100 ;
DUSP4 ;

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family , and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity , and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore , it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus , DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferati

Catalytic activity:A phosphoprotein + H (2) O = a protein + phosphate. ,Catalytic activity:Protein tyrosine phosphate + H (2) O = protein tyrosine + phosphate. ,Function:Dual specificity phosphatase that dephosphorylates MAP kinase ERK2 on both 'Thr-183' and 'Tyr-185'. ,induction:By oxidative stress and heat shock. ,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. ,similarity:Contains 1 rhodanese domain. ,similarity:Contains 1 tyrosine-protein phosphatase domain. ,tissue specificity:Expressed at high levels in the lung , liver placenta and pancreas. Moderate levels seen in the heart and skeletal muscle. Lower levels found in the brain and kidney. ,

Nucleus .

>>MAPK signaling pathway ;
>>Serotonergic synapse ;
>>Parkinson disease ;
>>Fluid shear stress and atherosclerosis