The antiserum was produced against synthesized peptide derived from human PKC thet around the phosphorylation site of Thr538. AA range:504-553

Phospho-PKC θ (T538) Polyclonal Antibody detects endogenous levels of PKC θ protein only when phosphorylated at T538.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):TNtFC

PRKCQ

Protein kinase C theta type

PRKCQ ;
PRKCT ;
Protein kinase C theta type ;
nPKC-theta

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters , a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1 , and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq , Jul 2008] ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,cofactor:Magnesium. ,Domain:The C1 domain , containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B) , is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. ,enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain) , Ser-676 (turn motif) and Ser-695 (hydrophobic region) , need to be phosphorylated for its full activation. ,Function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters , a class of tumor promoters. ,Function:This is a calcium-independent , phospholipid-dependent , serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR) -mediated T-cell activation , but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. ,PTM:Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. ,similarity:Belongs to the protein kinase superfamily. ,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. ,similarity:Contains 1 AGC-kinase C-terminal domain. ,similarity:Contains 1 C2 domain. ,similarity:Contains 1 protein kinase domain. ,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers. ,subunit:Interacts with TXNL2/PICOT. ,tissue specificity:Skeletal muscle , megakaryoblastic cells and platelets. ,

Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells , mostly localized in cytoplasm. In response to TCR stimulation , associates with lipid rafts and then localizes in the immunological synapse.

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