The antiserum was produced against synthesized peptide derived from human PAK3 around the phosphorylation site of Ser154. AA range:121-170

Phospho-PAKβ (S154) Polyclonal Antibody detects endogenous levels of PAKβ protein only when phosphorylated at S154.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):YMsFT

PAK3

Serine/threonine-protein kinase PAK 3

PAK3 ;
OPHN3 ;
Serine/threonine-protein kinase PAK 3 ;
Beta-PAK ;
Oligophrenin-3 ;
p21-activated kinase 3 ;
PAK-3

The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Apr 2016],

Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Disease:Defects in PAK3 are the cause of mental retardation X-linked type 30 (MRX30) [MIM:300558]; also called X-linked mental retardation type 47 (MRX47). Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.,enzyme regulation:Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-436 and allows the kinase domain to adopt an active structure.,Function:Key regulator of synapse formation and plasticity in the hippocampus.,PTM:Autophosphorylated when activated by CDC42/p21.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CRIB domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. Shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK.,tissue specificity:Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.,

Cytoplasm .

>>ErbB signaling pathway ;
>>Ras signaling pathway ;
>>Axon guidance ;
>>Focal adhesion ;
>>T cell receptor signaling pathway ;
>>Regulation of actin cytoskeleton ;
>>Pathogenic Escherichia coli infection ;
>>Salmonella infection ;
>>Human immunodeficiency virus 1 infection ;
>>Renal cell carcinoma