The antiserum was produced against synthesized peptide derived from human KSR around the phosphorylation site of Ser392. AA range:358-407

Phospho-Ksr-1 (S392) Polyclonal Antibody detects endogenous levels of Ksr-1 protein only when phosphorylated at S392.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):TEsVP

KSR1

Kinase suppressor of Ras 1

KSR1 ;
KSR ;
Kinase suppressor of Ras 1

caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. ,function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself , it has no demonstrated kinase activity. ,PTM:Phosphorylated on Ser-309 and , to a higher extent , on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells , phosphorylated KSR1 is cytoplasmic and in stimulated cells , dephosphorylated KSR1 is membrane-associated. ,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ,similarity:Contains 1 phorbol-ester/DAG-type zinc finger. ,similarity:Contains 1 protein kinase domain. ,subcellular location:In unstimulated cells , where the phosphorylated form is bound to a 14-3-3 protein , sequestration in the cytoplasm occurs. Following growth factor treatment , the protein is free for membrane translocation , and it moves from the cytoplasm to the cell periphery. ,subunit:Interacts with HSPCA/HSP90 , YWHAB/14-3-3 , CDC37 , MAP2K/MEK , MARK3 , PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK , in a Ras-dependent manner (By similarity) . The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. ,

Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. ,Function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself , it has no demonstrated kinase activity. ,PTM:Phosphorylated on Ser-309 and , to a higher extent , on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells , phosphorylated KSR1 is cytoplasmic and in stimulated cells , dephosphorylated KSR1 is membrane-associated. ,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ,similarity:Contains 1 phorbol-ester/DAG-type zinc finger. ,similarity:Contains 1 protein kinase domain. ,subcellular location:In unstimulated cells , where the phosphorylated form is bound to a 14-3-3 protein , sequestration in the cytoplasm occurs. Following growth factor treatment , the protein is free for membrane translocation , and it moves from the cytoplasm to the cell periphery. ,subunit:Interacts with HSPCA/HSP90 , YWHAB/14-3-3 , CDC37 , MAP2K/MEK , MARK3 , PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK , in a Ras-dependent manner (By similarity) . The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization. ,

Cytoplasm . Membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Cell projection , ruffle membrane . Endoplasmic reticulum membrane . In unstimulated cells , where the phosphorylated form is bound to a 14-3-3 protein , sequestration in the cytoplasm occurs. Following growth factor treatment , the protein is free for membrane translocation , and it moves from the cytoplasm to the cell periphery. .

>>Ras signaling pathway ;
>>C-type lectin receptor signaling pathway ;
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