The antiserum was produced against synthesized peptide derived from human Tyrosine Hydroxylase around the phosphorylation site of Ser40. AA range:41-90

Phospho-TH (S71) Polyclonal Antibody detects endogenous levels of TH protein only when phosphorylated at S71.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RQsLI

TH

Tyrosine 3-monooxygenase (Tyrosine 3-hydroxylase) (TH) ,Tyrosine Hydrolase

TH ;
TYH ;
Tyrosine 3-monooxygenase ;
Tyrosine 3-hydroxylase ;
TH

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines , hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq , Jul 2008] ,

Catalytic activity:L-tyrosine + tetrahydrobiopterin + O (2) = 3 ,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin. ,cofactor:Fe (2+) ion. ,Disease:Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions , often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia , it is an eminently treatable condition , due to a favorable response to L-DOPA. ,enzyme regulation:Phosphorylation leads to an increase in the catalytic activity. ,Function:Plays an important role in the physiology of adrenergic neurons. ,online information:Tyrosine hydroxylase entry ,pathway:Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. ,similarity:Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. ,tissue specificity:Mainly expressed in the brain and adrenal glands. ,

Cytoplasm , perinuclear region . Nucleus . Cell projection , axon . Cytoplasm . Cytoplasmic vesicle , secretory vesicle , synaptic vesicle . When phosphorylated at Ser-19 shows a nuclear distribution and when phosphorylated at Ser-31 as well at Ser-40 shows a cytosolic distribution (By similarity) . Expressed in dopaminergic axons and axon terminals. .

>>Tyrosine metabolism ;
>>Folate biosynthesis ;
>>Metabolic pathways ;
>>Dopaminergic synapse ;
>>Prolactin signaling pathway ;
>>Parkinson disease ;
>>Cocaine addiction ;
>>Amphetamine addiction ;
>>Alcoholism