The antiserum was produced against synthesized peptide derived from human MAPKAPK2 around the phosphorylation site of Thr334. AA range:300-349

Phospho-MAPKAPK-2 (T334) Polyclonal Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T334.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):PQtPL

MAPKAPK2

MAP kinase-activated protein kinase 2

MAPKAPK2 ;
MAP kinase-activated protein kinase 2 ;
MAPK-activated protein kinase 2 ;
MAPKAP kinase 2 ;
MAPKAP-K2 ;
MAPKAPK-2 ;
MK-2 ;
MK2

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase , this kinase is known to be involved in many cellular processes including stress and inflammatory responses , nuclear export , gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq , Jul 2008] ,

Catalytic activity:ATP + a protein = ADP + a phosphoprotein. ,enzyme regulation:Seems to be activated by two distinct pathways: the first involves the stimulation of p42/p44 MAPK by growth factors , the second , triggered by stress and heat shock , depends on the activation of MPK2 and upstream MAPKK/MAPKKK. ,Function:Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25) . In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40') . This kinase phosphorylates Ser in the peptide sequence , Hyd-X-R-X (2) -S , where Hyd is a large hydrophobic residue (By similarity) . Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity. ,PTM:Phosphorylated and activated by MAP kinase. ,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. ,similarity:Contains 1 protein kinase domain. ,subunit:Interacts with PHC2. ,tissue specificity:Expressed in all tissues examined. ,

Cytoplasm . Nucleus . Phosphorylation and subsequent activation releases the autoinhibitory helix , resulting in the export from the nucleus into the cytoplasm.

>>MAPK signaling pathway ;
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>>VEGF signaling pathway ;
>>C-type lectin receptor signaling pathway ;
>>Neurotrophin signaling pathway ;
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>>Viral carcinogenesis